The experienced midfielder was forced off in the first half of Saturday's home defeat to Cardiff after aggravating a groin injury.
Kevin Blackwell will name two youngsters on the bench for the first-round clash against Chester tomorrow.
Danny Rose and Jonathan Howson could make their first senior appearances with former Wealdstone striker Jermaine Beckford also expected to be handed a run-out.
On-loan Fulham goalkeeper Tony Warner does not have permission to play, but Neil Sullivan has shaken off a leg injury and will make his first start of the season.
Former Australian international defender Hayden Foxe is likely to make his United debut at the heart of the defence.