The Aussie suffered a hamstring strain in training and is currently undergoing treatment.
Viduka has an impressive scoring record against the Old Trafford outfit, scoring four goals in six games against them.
Michael Bridges was left out of the reserve side which lost 3-1 to Newcastle last night and will be on stand-by if Viduka fails to shake off the injury.
The Whites are definitely without the suspended Ian Harte on Saturday and, of course, Jody Morris is serving a 14-day internal suspension with the club, but Brazilian Roque Junior is back in contention after serving a one-match ban.